美国hausser nageotte chamber计数板 上海拜普发展实业有限公司-凯发app官方网站

产品特点：

l  美国hausser scientific血球计数板，霍华德霉菌计数板，准确计算白细胞、红细胞、血小板、组织培养细胞、细菌和精子

l  the hausser nageotte bright-line hemacytometer allows one to manually count low leukocyte concentrations below the capabilities of a standard hemacytometer. the nageotte contains two gridded counting areas each containing 40 rectangular areas. total volume of each grid is 50 ml for a total volume of 100 ml per nageotte. the nageotte can accurately measure down to 0.1 cell/ml (100 cell/ml). cell depth is 0.5mm .

l  available with an h-shaped moat(catalog#4000) that separates the two gridded areas or without a moat (catalog#4001). the nageotte chamber comes with two reusable cover slips(30mm x 33mm x 0.5mm) and 1/2 oz (approx. 35 pcs.) of disposable cover slips (30mm x 33mm x 0.15mm).

l  process:  counting low levels of white cells in white cell-reduced plateletcomponents.

l  background: determination of the white cell (wbc) count in wbc-reduced platelet components requires methods that have a detection limit in the range of approximately 5.0 x 10(2) to 5.0 x 10(4) per ml.

l  study design and methods: with a 50-microl nageotte hemocytometer and bright-field microscopy (200x magnification), studies were conducted to develop and validate a method that could be used routinely with filtered and apheresis-harvested platelets. a 1-in-5 dilution of sample with a commercially available blood-diluting fluid was used because, with a lower (1-in-2) dilution, the observed number of wbcs was substantially less than the number expected at relatively high platelet counts (> 1.9 x 10(9)/ml).

l  results: the observed and expected wbc counts in wbc-reduced platelet samples correlated well at levels between approximately 5 and 1100 wbcs per counting area (5.0 x 10(2)-1.1 x 10(5)/ml). at levels of more than 300 to 400 wbcs per counting area, accurate counts were obtained when 10 of the 40 rectangles were counted.

l  conclusion: these studies provide data to confirm that the 50-microl nageotte hemocytometer can be used to accurately count low levels of wbcs in platelet components.